T7 Promoter Induction
MULTIPLE STRATEGIES TO IMPROVE EXTRACELLULAR SECRETION AND …
Updated 55 years ago
18 hours ago Furthermore, by cascading T7 promoters, replacing the 5′ UTR, randomly mutating the N-terminal sequence, and co-expressing secretory cofactors, the … ...
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BIOSENSOR THAT DETECTS STRESS CAUSED BY PERIPLASMIC …
Updated 55 years ago
5 days ago After 20 h of induction, ... ) has also been used previously to amplify the output signal from the T7 and pBAD promoters. We anticipate that it will be useful for … ...
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Get CodeFOUR PHOSPHOLIPASE A2 GENES ENCODED IN THE WESTERN …
Updated 55 years ago
4 days ago To prepare dsRNAs specific to different genes, template DNAs were amplified with forward and reverse gene-specific primers containing the T7 promoter sequence at … ...
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Get CodePRECISE MICROBIOME ENGINEERING USING NATURAL AND …
Updated 55 years ago
1 day ago The T7 phage was obtained from the Biological Research Center, NITE. ... and 10 mM arabinose for the induction of λ-Red recombinase. Once the OD 600 reached … ...
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Get CodeTARGETED MUTAGENESIS | SPRINGERLINK
Updated 55 years ago
18 hours ago Cas9 cleaves the target DNA adjacent to the PAM (NGG) motif. (b) The sgRNA template consists of a T7 promoter, target site, and sg RNA sequence. Two … ...
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MECHANISTIC MODELING OF IN VITRO TRANSCRIPTION INCORPORATING EFFECTS …
Updated 55 years ago
1 day ago A typical IVT scheme requires a linearized template DNA of the target sequence, NTPs, T7 RNA polymerase (T7 RNAP), and a magnesium salt in an aqueous … ...
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Get CodeGENOME-WIDE IDENTIFICATION AND EXPRESSION ANALYSIS OF …
Updated 55 years ago
18 hours ago The objectives of our study can be summarized as in silico analysis to (i) identify StSET genes in the potato genome, (ii) systematically analyse gene structure, … ...
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Get CodePROBING PAC1 RECEPTOR ACTIVATION ACROSS SPECIES WITH AN …
Updated 55 years ago
1 day ago AAVs encoding the PAClight1 P78A or PAClight1 P78A-ctrl sensor under control of a human synapsin-1 promoter were ... An individual colony was manually selected … ...
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Get CodeIMMUNE SIGNALING OF LITOPENAEUS VANNAMEI C-TYPE …
Updated 55 years ago
4 days ago To prepare specific dsRNAs for the gene knockdown experiment, fragments of LvTLR2 and LvDOME were synthesized by T7 RiboMAX Express RNAi System kit … ...
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NON-CODING RNAS FUNCTION AS DIAGNOSTIC BIOMARKERS AND
Updated 55 years ago
4 days ago The combination of miR-17/18a/19a/92a demonstrates the most potent induction effect on PCNA and α-SMA, while maintaining calponin and SM22α levels. ... ...
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Get CodeAMERICAN ASSOCIATION FOR CANCER RESEARCH
Updated 55 years ago
1 day ago The pcDNA3.1-MILIP, pcDNA3.1-MILIP-ΔE1, pcDNA3.1-MILIP-ΔE2, pcDNA3.1-MILIP-Δ-990/-1895, pcDNA3.1-MILIP-Δ-1488/-1895 plasmids were linearized using … ...
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Get CodeLENTIVIRAL VECTOR FOR GENE EXPRESSION | VECTORBUILDER
Updated 55 years ago
3 days ago Customizable internal promoter: Our vector is designed to self-inactivate the promoter activity in its 5' LTR upon integration into the genome. As a result, users can … ...
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Get CodeUTILIZING CHITOOLIGOSACCHARIDES FROM SHRIMP WASTE BIODEGRADATION …
Updated 55 years ago
1 day ago Background Hydrocarbon pollution stemming from petrochemical activities is a significant global environmental concern. Bioremediation, employing microbial chitinase … ...
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REGULAR PLASMID GENE EXPRESSION VECTOR | VECTORBUILDER
Updated 55 years ago
4 days ago Promoter: The promoter that drives your gene of interest is placed here. Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of … ...
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Get CodeREPRESSOR - NATIONAL HUMAN GENOME RESEARCH INSTITUTE
Updated 55 years ago
4 days ago A repressor, as related to genomics, is a protein that inhibits the expression of one or more genes. The repressor protein works by binding to the promoter region of … ...
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Get CodeLESSON EXPLAINER: CLONING DNA SEQUENCES | NAGWA
Updated 55 years ago
4 days ago DNA cloning is the process of making more copies of a particular segment of DNA. In order to clone DNA into bacteria, we first need to place the DNA into specialized … ...
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Get CodeYEAST RECOMBINANT PROTEIN EXPRESSION VECTOR | VECTORBUILDER
Updated 55 years ago
3 days ago This vector system is designed for either constitutive or inducible expression of recombinant proteins in P. pastoris. The gene of interest is cloned into the vector under … ...
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CYP3A4 - WIKIPEDIA
Updated 55 years ago
1 day ago Induction. CYP3A4 is induced by a wide variety of ligands. These ligands bind to the pregnane X receptor (PXR). The activated PXR complex forms a heterodimer with … ...
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Get CodeINDUCIBLE GENE EXPRESSION VECTOR (TET-ON) | VECTORBUILDER
Updated 55 years ago
4 days ago Our Tet-On inducible gene expression vectors are designed to achieve nearly complete silencing of a GOI in the absence of tetracycline and its analogs (e.g. … ...
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Get CodeAVOID EXPLOSION OF INDUCTION COOKER AT HOME, PLEASE PAY ... - 資訊咖
Updated 55 years ago
5 days ago The induction cooker uses the induction principle of the induction cooker. Induction of the furnace surface is heat-resistant ceramic plates , alternating current by … ...
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Get CodeREGULAR PLASMID ENHANCER TESTING VECTOR | VECTORBUILDER
Updated 55 years ago
4 days ago Our vector is based on a regular plasmid system. The putative enhancer to be tested is placed immediately upstream of a minimal promoter, which controls the … ...
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INDUCTION TABLETOP SINGLE BURNER - PARTY RENTAL LTD.
Updated 55 years ago
5 days ago Helpful Hints. Requires induction pan. Maximum temperature for burner is 200 degrees. Find the perfect Induction Tabletop Single Burner from Party Rental Ltd. … ...
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Get CodePROMOTERS FOR GENE EXPRESSION | VECTORBUILDER
Updated 55 years ago
3 days ago Strong and heat-shock inducible promoter in somatic cells and germline. We offer a variety of promoters to drive target gene expression including promoters for … ...
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