T7 Promoter Induction
MULTIPLE STRATEGIES TO IMPROVE EXTRACELLULAR SECRETION AND ACTIVITY …
Updated 55 years ago
18 hours ago Furthermore, by cascading T7 promoters, replacing the 5′ UTR, randomly mutating the N-terminal sequence, and co-expressing secretory cofactors, the extracellular secretion was increased by 36.46 %, 31.25 %, 20.66 % and 25.75 %, respectively. Moreover, the feruloyl esterase were mutated to improve the substrate affinity and activity. ...
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BIOSENSOR THAT DETECTS STRESS CAUSED BY PERIPLASMIC PROTEINS
Updated 55 years ago
5 days ago After 20 h of induction, ... ) has also been used previously to amplify the output signal from the T7 and pBAD promoters. We anticipate that it will be useful for other transcription-based biosensors. The study noted three limitations with the [P … ...
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Get CodeFOUR PHOSPHOLIPASE A2 GENES ENCODED IN THE WESTERN FLOWER
Updated 55 years ago
4 days ago To prepare dsRNAs specific to different genes, template DNAs were amplified with forward and reverse gene-specific primers containing the T7 promoter sequence at their 5ʹ ends. ...
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Get CodePRECISE MICROBIOME ENGINEERING USING NATURAL AND SYNTHETIC ...
Updated 55 years ago
1 day ago The T7 phage was obtained from the Biological Research Center, NITE. ... and 10 mM arabinose for the induction of λ-Red recombinase. Once the OD 600 reached 0.5, competent cells were prepared ... as a rebooting host. However, it will be difficult to reboot all phages using a single host strain because phage promoter must be recognized by … ...
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Get CodeTARGETED MUTAGENESIS | SPRINGERLINK
Updated 55 years ago
18 hours ago Cas9 cleaves the target DNA adjacent to the PAM (NGG) motif. (b) The sgRNA template consists of a T7 promoter, target site, and sg RNA sequence. Two primers (F and R) are used to PCR amplify the template. sgRNA is synthesized by T7 RNA polymerase. ... Split Cas9 is activated by chemical or optical induction of a heterodimer … ...
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MECHANISTIC MODELING OF IN VITRO TRANSCRIPTION INCORPORATING EFFECTS …
Updated 55 years ago
1 day ago A typical IVT scheme requires a linearized template DNA of the target sequence, NTPs, T7 RNA polymerase (T7 RNAP), and a magnesium salt in an aqueous buffered reaction at 37 ° $37^\circ $ C and a pH around 7.5–8 (Beckert & Masquida, 2011). In addition, many IVT reaction schemes include PPiase, surfactants, spermadine, and … ...
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Get CodeGENOME-WIDE IDENTIFICATION AND EXPRESSION ANALYSIS OF THE SET …
Updated 55 years ago
18 hours ago The objectives of our study can be summarized as in silico analysis to (i) identify StSET genes in the potato genome, (ii) systematically analyse gene structure, chromosomal distribution, gene duplication events, promoter sequences, and protein domains, (iii) perform phylogenetic analyses, (iv) compare the SET domain-containing … ...
Category: Domain
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Get CodePROBING PAC1 RECEPTOR ACTIVATION ACROSS SPECIES WITH AN ... - ELIFE
Updated 55 years ago
1 day ago AAVs encoding the PAClight1 P78A or PAClight1 P78A-ctrl sensor under control of a human synapsin-1 promoter were ... An individual colony was manually selected and expanded for subsequent experiments. Induction of ... -540nm). Signals from the photodetectors were amplified with Dorics built-in amplifiers and acquired using a … ...
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Get CodeIMMUNE SIGNALING OF LITOPENAEUS VANNAMEI C-TYPE LYSOZYME AND …
Updated 55 years ago
4 days ago To prepare specific dsRNAs for the gene knockdown experiment, fragments of LvTLR2 and LvDOME were synthesized by T7 RiboMAX Express RNAi System kit (Promega, USA) using gene-specific primers flanked with the T7 promoter sequence . GFP was used as a control. The quality of dsRNA was verified after annealing by agarose gel … ...
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NON-CODING RNAS FUNCTION AS DIAGNOSTIC BIOMARKERS AND
Updated 55 years ago
4 days ago The combination of miR-17/18a/19a/92a demonstrates the most potent induction effect on PCNA and α-SMA, while maintaining calponin and SM22α levels. ... PiRNA-63076 partially inhibits the expression of Acadm by mediating DNA methylation in the promoter region of Acadm, ... Tat and T7 peptides, (b) NT-lipidoids, and (c) aptamer … ...
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Get CodeAMERICAN ASSOCIATION FOR CANCER RESEARCH
Updated 55 years ago
1 day ago The pcDNA3.1-MILIP, pcDNA3.1-MILIP-ΔE1, pcDNA3.1-MILIP-ΔE2, pcDNA3.1-MILIP-Δ-990/-1895, pcDNA3.1-MILIP-Δ-1488/-1895 plasmids were linearized using restriction enzyme BstBI (New England Biolab, #R0519S) and in vitro transcription was conducted using a TranscriptAid T7 High Yield Transcription Kit (Thermo Fisher … ...
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Get CodeLENTIVIRAL VECTOR FOR GENE EXPRESSION | VECTORBUILDER
Updated 55 years ago
3 days ago Customizable internal promoter: Our vector is designed to self-inactivate the promoter activity in its 5' LTR upon integration into the genome. As a result, users can put in their own promoter to drive their gene of interest within the vector. ...
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Get CodeUTILIZING CHITOOLIGOSACCHARIDES FROM SHRIMP WASTE BIODEGRADATION …
Updated 55 years ago
1 day ago Background Hydrocarbon pollution stemming from petrochemical activities is a significant global environmental concern. Bioremediation, employing microbial chitinase-based bioproducts to detoxify or remove contaminants, presents an intriguing solution for addressing hydrocarbon pollution. Chitooligosaccharides, a product of chitin degradation … ...
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REGULAR PLASMID GENE EXPRESSION VECTOR | VECTORBUILDER
Updated 55 years ago
4 days ago Promoter: The promoter that drives your gene of interest is placed here. Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of interest because it is believed to facilitate translation initiation in eukaryotes. ...
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Get CodeREPRESSOR - NATIONAL HUMAN GENOME RESEARCH INSTITUTE
Updated 55 years ago
4 days ago A repressor, as related to genomics, is a protein that inhibits the expression of one or more genes. The repressor protein works by binding to the promoter region of the gene (s), which prevents the production of messenger RNA (mRNA). Repressor proteins are essential for the regulation of gene expression in cells. Narration. 00:00. … Repressor. ...
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Get CodeLESSON EXPLAINER: CLONING DNA SEQUENCES | NAGWA
Updated 55 years ago
4 days ago DNA cloning is the process of making more copies of a particular segment of DNA. In order to clone DNA into bacteria, we first need to place the DNA into specialized carrier DNA. This is called a vector and is a piece of DNA that acts as a vehicle to transport DNA into a cell. ...
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Get CodeYEAST RECOMBINANT PROTEIN EXPRESSION VECTOR | VECTORBUILDER
Updated 55 years ago
3 days ago This vector system is designed for either constitutive or inducible expression of recombinant proteins in P. pastoris. The gene of interest is cloned into the vector under the control of either the constitutive GAP promoter or the methanol-inducible AOX1 promoter. ...
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CYP3A4 - WIKIPEDIA
Updated 55 years ago
1 day ago Induction. CYP3A4 is induced by a wide variety of ligands. These ligands bind to the pregnane X receptor (PXR). The activated PXR complex forms a heterodimer with the retinoid X receptor (RXR), which binds to the XREM region of the CYP3A4 gene. ...
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Get CodeINDUCIBLE GENE EXPRESSION VECTOR (TET-ON) | VECTORBUILDER
Updated 55 years ago
4 days ago Our Tet-On inducible gene expression vectors are designed to achieve nearly complete silencing of a GOI in the absence of tetracycline and its analogs (e.g. doxycycline), and strong, rapid expression in response to the addition of tetracycline or one of its analogs (e.g. doxycycline). ...
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Get CodeAVOID EXPLOSION OF INDUCTION COOKER AT HOME, PLEASE PAY ... - 資訊咖
Updated 55 years ago
5 days ago The induction cooker uses the induction principle of the induction cooker. Induction of the furnace surface is heat-resistant ceramic plates , alternating current by generating a magnetic field coil beneath the ceramic plate , the magnetic force of the magnetic field when the iron is the object , will react with the iron , and high-speed heating . ...
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Get CodeREGULAR PLASMID ENHANCER TESTING VECTOR | VECTORBUILDER
Updated 55 years ago
4 days ago Our vector is based on a regular plasmid system. The putative enhancer to be tested is placed immediately upstream of a minimal promoter, which controls the expression of a downstream reporter gene. An active enhancer would stimulate the minimal promoter, driving reporter gene expression. ...
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INDUCTION TABLETOP SINGLE BURNER - PARTY RENTAL LTD.
Updated 55 years ago
5 days ago Helpful Hints. Requires induction pan. Maximum temperature for burner is 200 degrees. Find the perfect Induction Tabletop Single Burner from Party Rental Ltd. Browse all party rental equipment like linens, flatware & … ...
Category: Degree
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Get CodePROMOTERS FOR GENE EXPRESSION | VECTORBUILDER
Updated 55 years ago
3 days ago Strong and heat-shock inducible promoter in somatic cells and germline. We offer a variety of promoters to drive target gene expression including promoters for mammalian, plant, zebrafish, yeast and drosophila gene expression. ...
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